Roller Organs were not tuned to standard pitch, so there is great variation. A scale beginning on D will allow D, G, A, C, and E major chords and D, G, and A scales, so it is well-suited to play in the key of D or G. The mean temperament tuning1) allows it to play in almost perfect harmony.
20-Note Scale:
D G A B (middle)C C# D E F# G G# A B C C# D E F# G A 2)
B E F# G# A A# B C# D# E F F# G# A A# B C# D# E F# 3)
C# F# G# A# B (middle)C C# D# F F# G G# A# B C C# D# F F# G# 4)
D# G# A# C C# D D# F G G# A A# C C# D D# F G G# A# 5)
1)
Mean temperament was used prior to 1908 (approximately). At that time the Autophone Company switched to equal temperament because the reed sets were slightly less expensive.